DNA and RNA sequencing is the process of determining the nucleotide order of a given DNA fragment. The nucleotide sequence encodes the necessary information that allows living things to survive and reproduce. Determining the sequence is therefore useful in researching into how organisms live. Our core facility provides many sequencing options to meet your individual needs, utilizing two different NGS platforms.
The human genome contains approximately 20,000 genes, and each cell of the human body contains a copy ofevery gene.At a given moment, each cell has a combination of genes that are turned on and off. This combination of genes that "on/off" can change at any time depending on internal and external stimuli.Thus, it can be very important and telling to determine which genes are turned on and off. Microarray analysis allows us to do this. It involves breaking open a cell, isolating its genetic contents, identifying all the genes that are turned on in that particular cell, and generating a list of those genes. Our core facility is equipped with various platforms to meet your research needs.
Our core facility has various academic and commercial data mining software for genomic data analysis, including Partek Genomics Suite, NextGENe, IPA and Hoffman2 data analysis and storage server.
Educational workshops are offered annually. Our core facility also provides RNA/DNA extraction; RNA/DNA quality control and quantification; cDNA synthesis for quantitative-PCR; and QRT-PCR.